DETERMINATION OF NICOTINIC ACID

The Microbiological Determination of Nicotinic Acid, Nicotinamide, and Nicotinuric Acid

The nicotinic acid assay most generally satisfactory is the Lactobacillus arabinosus procedure (1, 2) as regards sensitivity and non-interference from other compounds. However, this organism responds to nicotinamide and nicotinuric acid as well as to nicotinic acid. Gaines and Stahly (3) have proposed the use of Leuconostoc mesenteroides for the assay of several members of the vitamin B complex...

Determination of cellular nicotinic acid-adenine dinucleotide phosphate (NAADP) levels.

Nicotinic acid-adenine dinucleotide phosphate (NAADP) is fast emerging as a new intracellular Ca2+-mobilizing messenger. In sea urchin egg homogenates, binding of NAADP to its receptor is not readily reversible; hence, prior incubation with low concentrations of NAADP is more effective in inhibiting subsequent binding of radiolabelled NAADP than incubating the preparation with the two ligands s...

Simultaneous spectrophotometric determination of trigonelline, diosgenin and nicotinic acid in dosage forms prepared from fenugreek seed extract

Mathematical algorithms offer a useful method for quantitative analysis of compounds in multi-component mixtures to overcome the overlapping problems occurred in UV spectrophotometry. The aim of this study is to develop a method to determine the bioactive compounds in herbal dosage forms produced from fenugreek extract. A UV- spectrophotometric method based on mathematical algorithm was used to...

Simultaneous spectrophotometric determination of trigonelline, diosgenin and nicotinic acid in dosage forms prepared from fenugreek seed extract

Mathematical algorithms offer a useful method for quantitative analysis of compounds in multi-component mixtures to overcome the overlapping problems occurred in UV spectrophotometry. The aim of this study is to develop a method to determine the bioactive compounds in herbal dosage forms produced from fenugreek extract. A UV- spectrophotometric method based on mathematical algorithm was used to...

Experience with Nicotinic Acid